Ordering BAC Clones from Fingerprint Data obtained through Four Enzymes Digestion and Fluorescent Labeling
نویسندگان
چکیده
We implement a solution for the automatic ordering of BAC clones from fingerprint data obtained through full digestion of the clones with four enzymes that leave different 3’ recessed ends and fluorescent labeling. The algorithm is inspired to a previous existing approach for building restriction maps.
منابع مشابه
High-throughput fingerprinting of bacterial artificial chromosomes using the snapshot labeling kit and sizing of restriction fragments by capillary electrophoresis.
We have developed an automated, high-throughput fingerprinting technique for large genomic DNA fragments suitable for the construction of physical maps of large genomes. In the technique described here, BAC DNA is isolated in a 96-well plate format and simultaneously digested with four 6-bp-recognizing restriction endonucleases that generate 3' recessed ends and one 4-bp-recognizing restriction...
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